Maxi Fast-Ion Plasmid Kit
Efficient Maxi Kits for High-Quality Plasmid Purification
The Fast-Ion Maxi Kits are designed for the efficient purification of plasmid or cosmid DNA from bacterial cultures, utilizing pre-packed ion-exchange resin columns for reliable results. This kit employs a modified alkaline lysis method combined with RNase treatment to produce a cleared lysate with minimal genomic DNA and RNA contamination. Through a straightforward gravity-flow procedure, plasmid DNA binds to the column while contaminants are washed away with a specially formulated buffer. The purified DNA is then eluted using a high-salt buffer and precipitated with isopropanol, ensuring a clean, high-yield product in under two hours.
High-Performance Solutions for Plasmid Applications
Purified plasmid DNA from the Fast-Ion Maxi Kits is ideal for applications such as transfection, PCR, sequencing, and in-vitro transcription. Each lot undergoes rigorous quality testing by isolating plasmid DNA from 100 ml of E. coli cultures, with yields exceeding 400 µg per preparation. This ensures consistent, high-quality DNA with excellent performance in downstream applications. The kit supports both high-copy plasmids (100 ml cultures) and low-copy plasmids (250 ml cultures), delivering yields of 500 µg to 1 mg of DNA per prep. With its user-friendly operation and dependable results, the Maxi Kit is an essential tool for plasmid purification.
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Maxi Fast-Ion Plasmid Kit
Efficient Maxi Kits for High-Quality Plasmid Purification
The Fast-Ion Maxi Kits are designed for the efficient purification of plasmid or cosmid DNA from bacterial cultures, utilizing pre-packed ion-exchange resin columns for reliable results. This kit employs a modified alkaline lysis method combined with RNase treatment to produce a cleared lysate with minimal genomic DNA and RNA contamination. Through a straightforward gravity-flow procedure, plasmid DNA binds to the column while contaminants are washed away with a specially formulated buffer. The purified DNA is then eluted using a high-salt buffer and precipitated with isopropanol, ensuring a clean, high-yield product in under two hours.
High-Performance Solutions for Plasmid Applications
Purified plasmid DNA from the Fast-Ion Maxi Kits is ideal for applications such as transfection, PCR, sequencing, and in-vitro transcription. Each lot undergoes rigorous quality testing by isolating plasmid DNA from 100 ml of E. coli cultures, with yields exceeding 400 µg per preparation. This ensures consistent, high-quality DNA with excellent performance in downstream applications. The kit supports both high-copy plasmids (100 ml cultures) and low-copy plasmids (250 ml cultures), delivering yields of 500 µg to 1 mg of DNA per prep. With its user-friendly operation and dependable results, the Maxi Kit is an essential tool for plasmid purification.
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Description
Efficient Maxi Kits for High-Quality Plasmid Purification
The Fast-Ion Maxi Kits are designed for the efficient purification of plasmid or cosmid DNA from bacterial cultures, utilizing pre-packed ion-exchange resin columns for reliable results. This kit employs a modified alkaline lysis method combined with RNase treatment to produce a cleared lysate with minimal genomic DNA and RNA contamination. Through a straightforward gravity-flow procedure, plasmid DNA binds to the column while contaminants are washed away with a specially formulated buffer. The purified DNA is then eluted using a high-salt buffer and precipitated with isopropanol, ensuring a clean, high-yield product in under two hours.
High-Performance Solutions for Plasmid Applications
Purified plasmid DNA from the Fast-Ion Maxi Kits is ideal for applications such as transfection, PCR, sequencing, and in-vitro transcription. Each lot undergoes rigorous quality testing by isolating plasmid DNA from 100 ml of E. coli cultures, with yields exceeding 400 µg per preparation. This ensures consistent, high-quality DNA with excellent performance in downstream applications. The kit supports both high-copy plasmids (100 ml cultures) and low-copy plasmids (250 ml cultures), delivering yields of 500 µg to 1 mg of DNA per prep. With its user-friendly operation and dependable results, the Maxi Kit is an essential tool for plasmid purification.
