Phosphate Buffered Saline (PBS) Solution: Essential for Laboratory Applications

Phosphate Buffered Saline (PBS) solution is a crucial component in many laboratory and cell culture applications. It is a balanced salt solution that provides an isotonic environment, making it ideal for washing cells, transporting tissues, diluting cells for counting, and preparing reagents. IBI Scientific's 10X PBS solution is specially formulated to exclude Magnesium, Calcium, Phenol Red, and Sodium Pyruvate, ensuring it remains compatible with a wide range of experimental applications.

The quality of PBS solution plays a significant role in laboratory research, as it is used in many critical procedures where consistency and purity are essential. IBI Scientific ensures that its PBS solution is manufactured under stringent conditions to meet the highest standards. It is prepared with Type 1 Nuclease Free and Endotoxin Free, Reagent Grade Water, which guarantees that the solution remains free from contaminants that could interfere with sensitive experiments.

Ultra-Pure and Sterile PBS Solution for Reliable Research

One of the defining characteristics of IBI Scientific’s PBS solution is its ultra-pure quality. The solution undergoes a rigorous filtration process using a 0.22-micron sterile filter, ensuring that all potential microbial contaminants are removed. This level of sterility is maintained throughout the production process, with aseptic filling taking place within our ISO 7 sterile suite. Every lot of PBS solution is subjected to strict quality control measures to verify sterility, and the results are documented on a Certificate of Analysis (C of A).

The composition of our PBS solution includes Sodium phosphate, Sodium chloride, Potassium phosphate, and Potassium chloride, creating a physiologically relevant buffer system that is widely used in biological research. Because it is formulated without Magnesium and Calcium, it minimizes the risk of unwanted interactions in experiments where these ions may interfere with cell behavior or enzymatic reactions. Additionally, the absence of Phenol Red ensures that it does not impact pH-dependent assays, making it suitable for a broad range of laboratory applications.

Versatile Applications of PBS Solution in Scientific Research

PBS solution serves as a foundational component in a variety of laboratory protocols. It is commonly used for washing cells in tissue culture, helping to remove excess serum, proteins, or metabolic byproducts without disrupting the cells. The balanced osmolarity and ion composition of PBS make it an effective solution for maintaining cell integrity during routine handling. Additionally, it is frequently used in immunohistochemistry, immunocytochemistry, and Western blotting as a washing buffer, ensuring optimal antibody binding and reducing background noise in assays.

Another critical application of PBS solution is in tissue transportation. When tissues or biopsy samples need to be transferred between labs or preserved for short-term storage, PBS provides an ideal medium to prevent desiccation and maintain physiological conditions. Furthermore, it is used for diluting and suspending cells for flow cytometry and cell counting applications, ensuring that samples remain evenly distributed and easy to analyze.

PBS also plays a key role in reagent preparation. Many biological and biochemical assays require precise dilution of proteins, enzymes, and antibodies, and PBS provides a stable environment for these reagents. Its pH buffering capacity helps maintain enzyme activity and protein stability, making it indispensable in molecular biology, protein chemistry, and microbiology workflows.

High-Quality Manufacturing Standards for Consistent Performance

IBI Scientific is committed to producing high-quality PBS solutions that meet the strictest standards for laboratory use. The solution is manufactured in an ISO 7 cleanroom facility, where it undergoes rigorous quality control at every stage of production. Each batch is tested for sterility, pH consistency, and endotoxin levels to ensure it meets industry requirements for use in cell culture and biochemical applications.

By using Type 1 Nuclease Free and Endotoxin Free, Reagent Grade Water , we eliminate potential contaminants that could interfere with experimental results. The sterile filtration process ensures that the PBS solution remains free from bacteria, fungi, and particulates, providing researchers with confidence in their experimental conditions.

Each lot of PBS solution is accompanied by a Certificate of Analysis, documenting the results of sterility testing and other quality control measures. This ensures traceability and provides researchers with the necessary documentation to validate the consistency and reliability of their reagents.

Why Choose IBI Scientific’s PBS Solution?

Researchers and laboratory professionals rely on IBI Scientific’s PBS solution for its purity, consistency, and versatility. With a formulation free from unwanted additives such as Magnesium, Calcium, and Phenol Red, it is optimized for a wide range of applications. The ultra-pure quality, combined with stringent sterility controls, makes it a trusted choice for cell culture, molecular biology, immunology, and diagnostic research.

The balanced composition of Sodium phosphate, Sodium chloride, Potassium phosphate, and Potassium chloride ensures that cells remain in a stable and physiologically relevant environment. Its use in tissue transport, reagent preparation, and cell washing applications highlights its essential role in laboratory workflows.

IBI Scientific’s PBS solution is designed to meet the needs of research professionals who require high-quality reagents for reproducible and reliable experiments. The careful selection of raw materials, precision manufacturing process, and comprehensive quality testing guarantee that each batch meets the highest standards.

Order Your PBS Solution Today

For laboratories seeking a high-purity, sterile, and reliable PBS solution, IBI Scientific offers the ideal choice. Whether you are conducting cell culture experiments, preparing biochemical assays, or transporting tissues, our PBS solution provides the consistency and quality you need. With strict manufacturing controls, ultra-pure reagent-grade water, and sterility assurance, you can trust our PBS solution to support your research endeavors.

Ensure your experiments are backed by high-quality reagents—order IBI Scientific’s PBS solution today and experience the difference in laboratory-grade reliability and performance.

PBS Collection

10X Sterile PBS

10X PBST (PBS w/0.5% TWEEN 20)

1X PBST (PBS w/0.05% TWEEN 20)